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Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-Beta catenin Polyclonal Antibody (bs-1165R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-Beta catenin Polyclonal Antibody (bs-1165R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-Beta catenin Polyclonal Antibody (bs-1165R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Beta catenin Polyclonal Antibody

BS-1165R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCTNNB1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Beta catenin Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1499
  • Target name
    CTNNB1
  • Target description
    catenin beta 1
  • Target synonyms
    armadillo; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta-1; CTNNB; EVR7; MRD19; NEDSDV
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35222
  • Protein Name
    Catenin beta-1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Porcine milk exosome miRNAs protect intestinal epithelial cells against deoxynivalenol-induced damage. Xie MY et al., 2020 May, Biochem Pharmacol
    Read more
  • MicroRNA-708 represses hepatic stellate cells activation and proliferation by targeting ZEB1 through Wnt/beta-catenin pathway. Yang J et al., 2020 Mar 15, Eur J Pharmacol
    Read more
  • lncRNA JPX/miR-33a-5p/Twist1 axis regulates tumorigenesis and metastasis of lung cancer by activating Wnt/beta-catenin signaling. Pan J et al., 2020 Jan 15, Mol Cancer
    Read more
  • Plant MIR156 regulates intestinal growth in mammals by targeting the Wnt/beta-catenin pathway. Li M et al., 2019 Sep 1, Am J Physiol Cell Physiol
    Read more
  • Inhibition of MRP4 alleviates sepsis-induced acute lung injury in rats. Xia W et al., 2019 Jul, Int Immunopharmacol
    Read more
  • A vanillin derivative suppresses the growth of HT29 cells through the Wnt/beta-catenin signaling pathway. Ma W et al., 2019 Apr 15, Eur J Pharmacol
    Read more
  • Hydroxylase Activity of ASPH Promotes Hepatocellular Carcinoma Metastasis Through Epithelial-to-Mesenchymal Transition Pathway. Zou Q et al., 2018 May, EBioMedicine
    Read more
  • Suppression of Wnt5a, but not Wnts, relieves chronic post-thoracotomy pain via anti-inflammatory modulation in rats. Zhu A et al., 2017 Nov 4, Biochem Biophys Res Commun
    Read more
  • Therapeutic role of human hepatocyte growth factor (HGF) in treating hair loss. Qi Y et al., 2016, PeerJ
    Read more
  • Lithium posttreatment confers neuroprotection through glycogen synthase kinase-3beta inhibition in intracerebral hemorrhage rats. Zheng J et al., 2017 Oct, J Neurosurg
    Read more

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Figure 1. Western blot analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human T47D whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNB1 antigen affinity purified polyclonal antibody (Catalog # A00004) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTNNB1 at approximately 95 kDa. The expected band size for CTNNB1 is at 95 kDa.
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