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Anti-beta catenin [15B8]

Ab01655-1.1
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, Other Application
Product group Antibodies
TargetCTNNB1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-beta catenin [15B8]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody is recommended for the detection and analysis of beta-catenin via various methods, such as Western blot, flow cytometry and immunofluorescence of cells and tissues. For instance, the antibody 15B8 was utilized in labelling beta-catenin in oral mucosa samples via immunohistochemistry (Das et al., 2013). Another group used this antibody in the identification of beta-catenin in extracts from K562 cells via Western blots (Kotula et al., 2014). Furthermore, this antibody was applied during immunohistochemistry stainings of transverse sections taken through the midbrain region of 8-9ss embryos (Schiffmacher et al., 2016). Finally,beta-catenin is a very popular marker used to outline the cell membrane, this antibody might be utilized for such purposes (Zhang et al., 2017).
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, Other Application
  • Applications Supplier
    WB; FC; IF; IHC; EMSA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    15B8
  • Gene ID1499
  • Target name
    CTNNB1
  • Target description
    catenin beta 1
  • Target synonyms
    armadillo; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta-1; CTNNB; EVR7; MRD19; NEDSDV
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP35222
  • Protein Name
    Catenin beta-1
  • Reactivity Supplier
    Human, Mouse, Rat, Cow, Dog, Chicken
  • Reactivity Supplier Note
    This antibody was raised by immunizing mice with a recombinant full length bèta-catenin of chicken origin.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human T47D whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNB1 antigen affinity purified polyclonal antibody (Catalog # A00004) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTNNB1 at approximately 95 kDa. The expected band size for CTNNB1 is at 95 kDa.
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