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Anti-CXCL13 [2C4]

AB03390-1.1-BT
Absolute Antibody
ApplicationsELISA, Other Application
Product group Antibodies
ReactivityMouse
TargetCxcl13
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CXCL13 [2C4]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The specificity of the original format of the antibody was confirmed by ELISA analysis (EC50 = 0.02 nM). The binding affinity of the IgG1 fragment for recombinant purified mouse CXCL13 was measured by surface plasmon resonance (Kd= 13 pM) (US20080227704).
  • Applications
    ELISA, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2C4
  • Gene ID55985
  • Target name
    Cxcl13
  • Target description
    C-X-C motif chemokine ligand 13
  • Target synonyms
    4631412M08Rik, ANGIE2, Angie, BCA-1, BLC, BLR1L, Scyb13, C-X-C motif chemokine 13, B lymphocyte chemoattractant, CXC chemokine BLC, chemokine (C-X-C motif) ligand 13, small inducible cytokine subfamily B (Cys-X-Cys), member 13, small-inducible cytokine B13
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO55038
  • Protein Name
    C-X-C motif chemokine 13
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CXCL13/BLC using anti-CXCL13/BLC antibody (PB9999). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant mouse CXCL13/BLC protein 1ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL13/BLC antigen affinity purified polyclonal antibody (Catalog # PB9999) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL13/BLC at approximately 13 kDa. The expected band size for CXCL13/BLC is at 12 kDa.
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