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ProductsBack/BCA1 antibody
Mouse BLC detected by Western blot in transfected supernatant.
Mouse BLC detected by Western blot in transfected supernatant.
Mouse BLC detected by Western blot in transfected supernatant.

BCA1 antibody

GTX53178
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityMouse
TargetCxcl13
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Overview

  • Supplier
    GeneTex
  • Product Name
    BCA1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    For WB: Use at a dilution of 1:5,000. Optimal dilutions/concentrations should be determined by the end user.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID55985
  • Target name
    Cxcl13
  • Target description
    C-X-C motif chemokine ligand 13
  • Target synonyms
    4631412M08Rik, ANGIE2, Angie, BCA-1, BLC, BLR1L, Scyb13, C-X-C motif chemokine 13, B lymphocyte chemoattractant, CXC chemokine BLC, chemokine (C-X-C motif) ligand 13, small inducible cytokine subfamily B (Cys-X-Cys), member 13, small-inducible cytokine B13
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO55038
  • Protein Name
    C-X-C motif chemokine 13
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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Figure 1. Western blot analysis of CXCL13/BLC using anti-CXCL13/BLC antibody (PB9999). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant mouse CXCL13/BLC protein 1ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL13/BLC antigen affinity purified polyclonal antibody (Catalog # PB9999) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL13/BLC at approximately 13 kDa. The expected band size for CXCL13/BLC is at 12 kDa.
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