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B-Lymphocyte Chemoattractant (BLC1) Polyclonal Antibody

CAU25647
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetCxcl13
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Overview

  • Supplier
    Biomatik
  • Product Name
    B-Lymphocyte Chemoattractant (BLC) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.24 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID55985
  • Target name
    Cxcl13
  • Target description
    chemokine (C-X-C motif) ligand 13
  • Target synonyms
    4631412M08Rik; ANG; Angie; ANGIE2; B lymphocyte chemoattractant; BCA-; BCA-1; BL; BLC; BLR1L; CXC chemokine BLC; C-X-C motif chemokine 13; Scyb1; Scyb13; small inducible cytokine subfamily B (Cys-X-Cys), member 13; small-inducible cytokine B13
  • Host
    Rabbit
  • Protein IDO55038
  • Protein Name
    C-X-C motif chemokine 13
  • Scientific Description
    The B-Lymphocyte Chemoattractant (BLC1) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CXCL13/BLC using anti-CXCL13/BLC antibody (PB9999). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant mouse CXCL13/BLC protein 1ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL13/BLC antigen affinity purified polyclonal antibody (Catalog # PB9999) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL13/BLC at approximately 13 kDa. The expected band size for CXCL13/BLC is at 12 kDa.
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