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Immunohistochemical staining of human rectum shows moderate positivity in plasma.
Immunohistochemical staining of human rectum shows moderate positivity in plasma.
Immunohistochemical staining of human rectum shows moderate positivity in plasma.

Anti-CXCL8 Antibody

HPA057179
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCXCL8
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CXCL8 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3576
  • Target name
    CXCL8
  • Target description
    C-X-C motif chemokine ligand 8
  • Target synonyms
    GCP-1, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8, interleukin-8, T-cell chemotactic factor, alveolar macrophage chemotactic factor I, beta endothelial cell-derived neutrophil activating peptide, beta-thromboglobulin-like protein, chemokine (C-X-C motif) ligand 8, emoctakin, granulocyte chemotactic protein 1, interleukin 8, lung giant cell carcinoma-derived chemotactic protein, lymphocyte derived neutrophil activating peptide, monocyte-derived neutrophil chemotactic factor, monocyte-derived neutrophil-activating peptide, neutrophil-activating peptide 1, small inducible cytokine subfamily B, member 8, tumor necrosis factor-induced gene 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10145
  • Protein Name
    Interleukin-8
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of IL-8 using anti-IL-8 antibody (PB9621). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: wild-type Hela whole cell lysate, Lane 2: Hela treated with TPA (400 ng/mL) for 4h whole cell lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-8 antigen affinity purified polyclonal antibody (Catalog # PB9621) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-8 at approximately 11 kDa. The expected band size for IL-8 is at 11 kDa.
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