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Formalin-fixed and paraffin embedded rabbit carotid artery labeled with Anti-IL-8/CXCL8 Polyclonal Antibody, Unconjugated (bs-0780R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rabbit carotid artery labeled with Anti-IL-8/CXCL8 Polyclonal Antibody, Unconjugated (bs-0780R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rabbit carotid artery labeled with Anti-IL-8/CXCL8 Polyclonal Antibody, Unconjugated (bs-0780R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

IL-8/CXCL8 Polyclonal Antibody

BS-0780R
Bioss
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityBovine, Human, Porcine, Rabbit, Sheep
TargetCXCL8
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Overview

  • Supplier
    Bioss
  • Product Name
    IL-8/CXCL8 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB(WB=1:500-2000), ELISA(ELISA=1:5000-10000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3576
  • Target name
    CXCL8
  • Target description
    C-X-C motif chemokine ligand 8
  • Target synonyms
    GCP-1, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8, interleukin-8, T-cell chemotactic factor, alveolar macrophage chemotactic factor I, beta endothelial cell-derived neutrophil activating peptide, beta-thromboglobulin-like protein, chemokine (C-X-C motif) ligand 8, emoctakin, granulocyte chemotactic protein 1, interleukin 8, lung giant cell carcinoma-derived chemotactic protein, lymphocyte derived neutrophil activating peptide, monocyte-derived neutrophil chemotactic factor, monocyte-derived neutrophil-activating peptide, neutrophil-activating peptide 1, small inducible cytokine subfamily B, member 8, tumor necrosis factor-induced gene 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10145
  • Protein Name
    Interleukin-8
  • Reactivity
    Bovine, Human, Porcine, Rabbit, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

References

  • The Effect of Low and High Dose Deoxynivalenol on Intestinal Morphology, Distribution, and Expression of Inflammatory Cytokines of Weaning Rabbits. Yang W et al., 2019 Aug 13, Toxins (Basel)
    Read this paper
  • Inflammatory cytokines tumour necrosis factor-alpha and interleukin-8 enhance airway smooth muscle contraction by increasing L-type Ca2+ channel expression. Ding S et al., 2019 Jan, Clin Exp Pharmacol Physiol
    Read this paper
  • IL-17 Promotes Angiogenic Factors IL-6, IL-8, and Vegf Production via Stat1 in Lung Adenocarcinoma. Huang Q et al., 2016 Nov 7, Sci Rep
    Read this paper
  • CXCL1 mediates obesity-associated adipose stromal cell trafficking and function in the tumour microenvironment. Zhang T et al., 2016 May 31, Nat Commun
    Read this paper
  • Extracts of Bauhinia championii (Benth.) Benth. attenuate the inflammatory response in a rat model of collagen-induced arthritis. Xu W et al., 2016 May, Mol Med Rep
    Read this paper

Datasheet

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Figure 1. Western blot analysis of IL-8 using anti-IL-8 antibody (PB9621). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: wild-type Hela whole cell lysate, Lane 2: Hela treated with TPA (400 ng/mL) for 4h whole cell lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-8 antigen affinity purified polyclonal antibody (Catalog # PB9621) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-8 at approximately 11 kDa. The expected band size for IL-8 is at 11 kDa.
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