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Western Blot analysis of HeLa cells using IL-8 Polyclonal Antibody
Western Blot analysis of HeLa cells using IL-8 Polyclonal Antibody
Western Blot analysis of HeLa cells using IL-8 Polyclonal Antibody

CXCL8 Antibody

CSB-PA003036
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCXCL8
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Overview

  • Supplier
    Cusabio
  • Product Name
    CXCL8 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3576
  • Target name
    CXCL8
  • Target description
    C-X-C motif chemokine ligand 8
  • Target synonyms
    GCP-1, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8, interleukin-8, T-cell chemotactic factor, alveolar macrophage chemotactic factor I, beta endothelial cell-derived neutrophil activating peptide, beta-thromboglobulin-like protein, chemokine (C-X-C motif) ligand 8, emoctakin, granulocyte chemotactic protein 1, interleukin 8, lung giant cell carcinoma-derived chemotactic protein, lymphocyte derived neutrophil activating peptide, monocyte-derived neutrophil chemotactic factor, monocyte-derived neutrophil-activating peptide, neutrophil-activating peptide 1, small inducible cytokine subfamily B, member 8, tumor necrosis factor-induced gene 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10145
  • Protein Name
    Interleukin-8
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IL-8 using anti-IL-8 antibody (PB9621). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: wild-type Hela whole cell lysate, Lane 2: Hela treated with TPA (400 ng/mL) for 4h whole cell lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-8 antigen affinity purified polyclonal antibody (Catalog # PB9621) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-8 at approximately 11 kDa. The expected band size for IL-8 is at 11 kDa.
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