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Anti-CXCR5 Antibody

Anti-CXCR5 Antibody

A9692
Antibodies.com
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-CXCR5 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to CXCR5.
  • Reactivity
    Human, Mouse
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CXCR5 using anti-CXCR5 antibody (A00663-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR5 antigen affinity purified polyclonal antibody (Catalog # A00663-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR5 at approximately 50 kDa. The expected band size for CXCR5 is at 42 kDa.
Product group Antibodies
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Anti-CXCR5 Antibody Picoband(r)A00663-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetCXCR5
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Product group Antibodies
RayBiotech
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Lane 1: Mouse lymph node lysates probed with CXCR5 Polyclonal Antibody, Unconjugated (bs-3598R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
Product group Antibodies
References
Bioss
CXCR5 Polyclonal AntibodyBS-3598R
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Human, Mouse, Porcine, Rabbit, Rat
TargetCXCR5
  • SizePrice
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA006255LA01HU at dilution of 1:100
Product group Antibodies
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ReactivityHuman
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  • SizePrice
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TargetCXCR5
  • SizePrice
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Boiled and unboiled Raji whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR5 antibody [HL3568] (GTX641474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
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TargetCXCR5
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