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Immunohistochemical staining of human adrenal gland shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows strong granular cytoplasmic positivity in glandular cells.

Anti-CYB5B Antibody

HPA007893
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetCYB5B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CYB5B
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80777
  • Target name
    CYB5B
  • Target description
    cytochrome b5 type B
  • Target synonyms
    CYB5-M; CYPB5M; cytochrome b5 type B; cytochrome b5 type B (outer mitochondrial membrane); OMB5; outer mitochondrial membrane cytochrome b5; type 2 cyt-b5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43169
  • Protein Name
    Cytochrome b5 type B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western blot All lanes: CYB5B antibody at 0.8microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Mouse liver tissue Lane 3: Mouse heart tissue Lane 4: Hela whole cell lysate Lane 5: U87 whole cell lysate Lane 6: EC109 whole cell lysate Lane 7: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 17 kDa Observed band size: 17 kDa
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Figure 1. Western blot analysis of CYB5B using anti-CYB5B antibody (A09841-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYB5B antigen affinity purified polyclonal antibody (Catalog # A09841-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYB5B at approximately 20 kDa. The expected band size for CYB5B is at 17 kDa.
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