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CYB5B Antibody

LS-C760836
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetCYB5B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CYB5B Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID80777
  • Target name
    CYB5B
  • Target description
    cytochrome b5 type B
  • Target synonyms
    CYB5-M, CYPB5M, OMB5, cytochrome b5 type B, cytochrome b5 type B (outer mitochondrial membrane), outer mitochondrial membrane cytochrome b5, type 2 cyt-b5
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western blot All lanes: CYB5B antibody at 0.8microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Mouse liver tissue Lane 3: Mouse heart tissue Lane 4: Hela whole cell lysate Lane 5: U87 whole cell lysate Lane 6: EC109 whole cell lysate Lane 7: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 17 kDa Observed band size: 17 kDa
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Figure 1. Western blot analysis of CYB5B using anti-CYB5B antibody (A09841-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYB5B antigen affinity purified polyclonal antibody (Catalog # A09841-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYB5B at approximately 20 kDa. The expected band size for CYB5B is at 17 kDa.
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