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Cyb5B Polyclonal Antibody

CAC11822
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetCYB5B
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Overview

  • Supplier
    Biomatik
  • Product Name
    Cyb5B Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80777
  • Target name
    CYB5B
  • Target description
    cytochrome b5 type B
  • Target synonyms
    CYB5-M, CYPB5M, OMB5, cytochrome b5 type B, cytochrome b5 type B (outer mitochondrial membrane), outer mitochondrial membrane cytochrome b5, type 2 cyt-b5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43169
  • Protein Name
    Cytochrome b5 type B
  • Scientific Description
    The Cyb5B Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
  • Reactivity
    Mouse
  • Reactivity Supplier
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CYB5B using anti-CYB5B antibody (A09841-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYB5B antigen affinity purified polyclonal antibody (Catalog # A09841-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYB5B at approximately 20 kDa. The expected band size for CYB5B is at 17 kDa.
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