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Immunofluorescent staining of human cell line MCF7 shows localization to nucleus.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleus.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleus.

Anti-DCK Antibody

HPA059609
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetDCK
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DCK
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1633
  • Target name
    DCK
  • Target description
    deoxycytidine kinase
  • Target synonyms
    deoxyadenosine kinase; deoxycytidine kinase; deoxyguanosine kinase; deoxynucleoside kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP27707
  • Protein Name
    Deoxycytidine kinase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DCK using anti-DCK antibody (A01655-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCK antigen affinity purified polyclonal antibody (Catalog # A01655-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DCK at approximately 31 kDa. The expected band size for DCK is at 31 kDa.
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