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Immunohistochemistry of paraffin-embedded human glioma using CSB-PA006547LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human glioma using CSB-PA006547LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human glioma using CSB-PA006547LA01HU at dilution of 1:100

DCK Antibody

CSB-PA006547LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetDCK
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Overview

  • Supplier
    Cusabio
  • Product Name
    DCK Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1633
  • Target name
    DCK
  • Target description
    deoxycytidine kinase
  • Target synonyms
    deoxycytidine kinase, deoxyadenosine kinase, deoxyguanosine kinase, deoxynucleoside kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP27707
  • Protein Name
    Deoxycytidine kinase
  • Scientific Description
    Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DCK using anti-DCK antibody (A01655-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCK antigen affinity purified polyclonal antibody (Catalog # A01655-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DCK at approximately 31 kDa. The expected band size for DCK is at 31 kDa.
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