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Immunohistochemical staining of human ovary shows moderate cytoplasmic positivity in stromal cells.
Immunohistochemical staining of human ovary shows moderate cytoplasmic positivity in stromal cells.
Immunohistochemical staining of human ovary shows moderate cytoplasmic positivity in stromal cells.

Anti-DCN Antibody

HPA003315
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetDCN
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DCN
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1634
  • Target name
    DCN
  • Target description
    decorin
  • Target synonyms
    bone proteoglycan II; CSCD; decorin; dermatan sulphate proteoglycans II; DSPG2; PG40; PGII; PGS2; proteoglycan core protein; SLRR1B; small leucine-rich protein 1B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07585
  • Protein Name
    Decorin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western blot All lanes: DCN antibody at 2microg/ml Lane 1: Mouse heart tissue Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40, 28, 24, 20, 9 kDa Observed band size: 40 kDa
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Immunohistochemistry analysis in human ovary and cerebral cortex tissues using Anti-DCN antibody. Corresponding DCN RNA-seq data are presented for the same tissues.
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Figure 1. Western blot analysis of Decorin using anti-Decorin antibody (PB9174). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: rat C6 whole cell lysates, Lane 9: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Decorin antigen affinity purified polyclonal antibody (Catalog # PB9174) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for Decorin at approximately 40-50KD and 80-140KD. The expected band sizes for Decorin are at 40-50KD and 80-140KD.
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