Anti-Desmoglein-3 [F779]

Absolute Antibody

Anti-Desmoglein-3 [F779]

7

This antibody can be used for the determination of desmoglein-3 using ELISA. The binding affinity of IgG1 version of this antibody for human desmoglein-3 was determined using surface plasmon resonance and was found to be Kd = 1.77 nM (PMID: 24942562). This antibody was also used in the direct and indirect immunofluorescence staining of frozen human skin sections (PMID: 24942562; 27304671) The IgG1 and IgG4 variants of this antibody induce loss of cell surface Dsg3 in primary human epidermal keratinocytes and suprabasal blisters in human skin (PMID: 27304671). This antibody is pathogenic in nature and caused suprabasal blisters in human skin at comparable doses of 25 microg and 50 microg (PMID: 27304671).

ImmunoFluorescence, ELISA

ELISA; IF

Research Use Only

Monoclonal

F779

DSG3

desmoglein 3

ABOLM, CDHF6, PVA, desmoglein-3, 130 kDa pemphigus vulgaris antigen, cadherin family member 6

Rabbit

IgG

Desmoglein-3

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.

Human

Human

The original antibody was generated from the peripheral blood of four patients with active untreated pemphigus vulgaris (PV) using heterohybridoma techniques.

-20°C,2°C to 8°C

12352203