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Anti-DGAT1 Antibody

144-06857
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetDGAT1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-DGAT1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8694
  • Target name
    DGAT1
  • Target description
    diacylglycerol O-acyltransferase 1
  • Target synonyms
    ACAT related gene product 1; acyl coenzyme A:cholesterol acyltransferase related gene 1; acyl-CoA retinol O-fatty-acyltransferase; acyl-CoA:diacylglycerol acyltransferase; ARAT; ARGP1; DGAT; diacylglycerol O-acyltransferase 1; DIAR7; diglyceride acyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75907
  • Protein Name
    Diacylglycerol O-acyltransferase 1
  • Scientific Description
    DGAT1 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DGAT1 using anti-DGAT1 antibody (PB9801). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Kidney Tissue Lysate at 50ug, Lane 2: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DGAT1 antigen affinity purified polyclonal antibody (Catalog # PB9801) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DGAT1 at approximately 60 kDa. The expected band size for DGAT1 is at 60 kDa.
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