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Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DGAT1 Polyclonal Antibody, Unconjugated (bs-2332R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DGAT1 Polyclonal Antibody, Unconjugated (bs-2332R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DGAT1 Polyclonal Antibody, Unconjugated (bs-2332R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

DGAT1 Polyclonal Antibody

BS-2332R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetDGAT1
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Overview

  • Supplier
    Bioss
  • Product Name
    Diglyceride acyltransferase Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID8694
  • Target name
    DGAT1
  • Target description
    diacylglycerol O-acyltransferase 1
  • Target synonyms
    ARAT, ARGP1, DGAT, DIAR7, diacylglycerol O-acyltransferase 1, ACAT related gene product 1, acyl coenzyme A:cholesterol acyltransferase related gene 1, acyl-CoA retinol O-fatty-acyltransferase, acyl-CoA:diacylglycerol acyltransferase, diglyceride acyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75907
  • Protein Name
    Diacylglycerol O-acyltransferase 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Increase of Autonomic Nerve Factors in Epicardial Ganglionated Plexi During Rapid Atrial Pacing Induced Acute Atrial Fibrillation. Li Y et al., 2017 Jul 27, Med Sci Monit
    Read more

Datasheet

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Figure 1. Western blot analysis of DGAT1 using anti-DGAT1 antibody (PB9801). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Kidney Tissue Lysate at 50ug, Lane 2: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DGAT1 antigen affinity purified polyclonal antibody (Catalog # PB9801) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DGAT1 at approximately 60 kDa. The expected band size for DGAT1 is at 60 kDa.
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