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Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA006817LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA006817LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA006817LA01HU at dilution of 1:100

DGAT1 Antibody

CSB-PA006817LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetDGAT1
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Overview

  • Supplier
    Cusabio
  • Product Name
    DGAT1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8694
  • Target name
    DGAT1
  • Target description
    diacylglycerol O-acyltransferase 1
  • Target synonyms
    ARAT, ARGP1, DGAT, DIAR7, diacylglycerol O-acyltransferase 1, ACAT related gene product 1, acyl coenzyme A:cholesterol acyltransferase related gene 1, acyl-CoA retinol O-fatty-acyltransferase, acyl-CoA:diacylglycerol acyltransferase, diglyceride acyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75907
  • Protein Name
    Diacylglycerol O-acyltransferase 1
  • Scientific Description
    Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. In liver, plays a role in esterifying exogenous fatty acids to glycerol. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DGAT1 Polyclonal Antibody, Unconjugated (bs-2332R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
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Figure 1. Western blot analysis of DGAT1 using anti-DGAT1 antibody (PB9801). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Kidney Tissue Lysate at 50ug, Lane 2: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DGAT1 antigen affinity purified polyclonal antibody (Catalog # PB9801) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DGAT1 at approximately 60 kDa. The expected band size for DGAT1 is at 60 kDa.
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