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Immunohistochemical staining of human uterus shows cytoplasmic immunoreactivity in the glandular epithelium cells.
Immunohistochemical staining of human uterus shows cytoplasmic immunoreactivity in the glandular epithelium cells.
Immunohistochemical staining of human uterus shows cytoplasmic immunoreactivity in the glandular epithelium cells.

Anti-DICER1 Antibody

AMAB90737
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetDICER1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DICER1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL0378
  • Conjugate
    Unconjugated
  • Gene ID23405
  • Target name
    DICER1
  • Target description
    dicer 1, ribonuclease III
  • Target synonyms
    aviD; DCR1; Dicer; dicer 1, double-stranded RNA-specific endoribonuclease; dicer 1, ribonuclease type III; Dicer1, Dcr-1 homolog; Dicer1e; endoribonuclease Dicer; GLOW; helicase MOI; helicase with RNAse motif; HERNA; K12H4.8-LIKE; MNG1; RMSE2
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ9UPY3
  • Protein Name
    Endoribonuclease Dicer
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Dicer/DICER1 using anti-Dicer/DICER1 antibody (A00190-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dicer/DICER1 antigen affinity purified polyclonal antibody (Catalog # A00190-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dicer/DICER1 at approximately 220-250 kDa. The expected band size for Dicer/DICER1 is at 219 kDa.
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