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Goat anti-Dicer1

EB06871
Everest Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetDICER1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Dicer1 Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID23405
  • Target name
    DICER1
  • Target description
    dicer 1, ribonuclease III
  • Target synonyms
    aviD; DCR1; Dicer; dicer 1, double-stranded RNA-specific endoribonuclease; dicer 1, ribonuclease type III; Dicer1, Dcr-1 homolog; Dicer1e; endoribonuclease Dicer; GLOW; helicase MOI; helicase with RNAse motif; HERNA; K12H4.8-LIKE; MNG1; RMSE2
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_085124.2; NP_803187.1. GeneIDs all Nonhuman: 192119 (mouse); 299284 (rat);. Purification: Antigen affinity purified. Names and symbols: DICER1; Dicer1, Dcr-1 homolog (Drosophila); HGNC:17098; Dicer; HERNA; KIAA0928 ; K12H4.8-LIKE; dicer1; helicase with RNAse motif; helicase-moi
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Dicer/DICER1 using anti-Dicer/DICER1 antibody (A00190-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dicer/DICER1 antigen affinity purified polyclonal antibody (Catalog # A00190-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dicer/DICER1 at approximately 220-250 kDa. The expected band size for Dicer/DICER1 is at 219 kDa.
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