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Anti-DLL3 [HP-92]

AB03572-23.159
Absolute Antibody
ApplicationsFlow Cytometry, ELISA
Product group Antibodies
TargetDLL3
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-DLL3 [HP-92]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The binding affinity to human DLL3 that is comparable with Rovalpituzumab. The binding characterization of this antibody for human DLL3 was done using ELISA. This antibody was capable of binding DLL3 expressed on the surface of H446 and H524 small cell lung cancer cell lines using flow cytometry (CN114057880).
  • Applications
    Flow Cytometry, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HP-92
  • Gene ID10683
  • Target name
    DLL3
  • Target description
    delta like canonical Notch ligand 3
  • Target synonyms
    delta3; delta-like 3; delta-like protein 3; drosophila Delta homolog 3; SCDO1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NYJ7
  • Protein Name
    Delta-like protein 3
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DLL3 using anti-DLL3 antibody (PA1912). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates Lane 2: mouse brain tissue lysates Lane 3: human HEK293 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLL3 antigen affinity purified polyclonal antibody (Catalog # PA1912) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLL3 at approximately 65KD. The expected band size for DLL3 is at 65KD.
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