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ICC/IF analysis of 4% PFA-fixed U2OS cells using GTX03439 DLL3 antibody. Green : Primary antibody Red : Actin Blue : DAPI Permeabilization : 0.1% Triton X-100 Dilution : 1:25
ICC/IF analysis of 4% PFA-fixed U2OS cells using GTX03439 DLL3 antibody. Green : Primary antibody Red : Actin Blue : DAPI Permeabilization : 0.1% Triton X-100 Dilution : 1:25
ICC/IF analysis of 4% PFA-fixed U2OS cells using GTX03439 DLL3 antibody. Green : Primary antibody Red : Actin Blue : DAPI Permeabilization : 0.1% Triton X-100 Dilution : 1:25

DLL3 antibody

GTX03439
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDLL3
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Overview

  • Supplier
    GeneTex
  • Product Name
    DLL3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:2000. ICC/IF: 1:25. IHC-P: 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10683
  • Target name
    DLL3
  • Target description
    delta like canonical Notch ligand 3
  • Target synonyms
    SCDO1, delta-like protein 3, delta-like 3, delta3, drosophila Delta homolog 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NYJ7
  • Protein Name
    Delta-like protein 3
  • Scientific Description
    This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostosis 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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IHC image of CSB-PA882142LA01HU diluted at 1:700 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of DLL3 using anti-DLL3 antibody (PA1912). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates Lane 2: mouse brain tissue lysates Lane 3: human HEK293 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLL3 antigen affinity purified polyclonal antibody (Catalog # PA1912) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLL3 at approximately 65KD. The expected band size for DLL3 is at 65KD.
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