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DLL3 Polyclonal Antibody

BS-7860R
Bioss Antibodies
ApplicationsWestern Blot, ELISA
DownloadDatasheet
Product group Antibodies
TargetDLL3
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    DLL3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID10683
  • Target name
    DLL3
  • Target description
    delta like canonical Notch ligand 3
  • Target synonyms
    delta3; delta-like 3; delta-like protein 3; drosophila Delta homolog 3; SCDO1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DLL3 using anti-DLL3 antibody (PA1912). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates Lane 2: mouse brain tissue lysates Lane 3: human HEK293 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLL3 antigen affinity purified polyclonal antibody (Catalog # PA1912) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLL3 at approximately 65KD. The expected band size for DLL3 is at 65KD.
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