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Anti-DLL4 [61B]

AB02657-2.3-BT
Absolute Antibody
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry, Neutralisation/Blocking
Product group Antibodies
TargetDLL4
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-DLL4 [61B]
  • Delivery Days Customer
    9
  • Application Supplier Note
    U87MG and HT29 cells were immune-stained with this antibody (Zhou et al, 2015; pmid:26288060). This antibody is used for immunohistochemistry on U87MG and HT29 (Zhou et al, 2015; pmid:26288060). The 64Cu-chelator DOTA conjugated version of this antibody was used for the positron-emission tomography (PET) imaging of human glioblastoma and colorectal cancer xenografts (Zhou et al, 2015; pmid:26288060). This antibody was used for immunohistochemistry on spheroids and monolayers cells (US7906116). ELISA was performed with this antibody on Dll4 truncation mutants (US7906116). This antibody is able to block the Dll4-Notch1 interaction (US7906116).
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    61B
  • Gene ID54567
  • Target name
    DLL4
  • Target description
    delta like canonical Notch ligand 4
  • Target synonyms
    AOS6; delta 4; delta ligand 4; delta4; delta-like 4 homolog; delta-like 4 protein; delta-like protein 4; drosophila Delta homolog 4; hdelta2; notch ligand delta-2; notch ligand DLL4
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ9NR61
  • Protein Name
    Delta-like protein 4
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DLL4 using anti-DLL4 antibody (A00875-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLL4 antigen affinity purified polyclonal antibody (Catalog # A00875-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLL4 at approximately 80 kDa. The expected band size for DLL4 is at 75 kDa.
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