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Anti-DLL4 APC

1A-717
EXBIO
ApplicationsFlow Cytometry
Product group Antibodies
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Overview

  • Supplier
    EXBIO
  • Product Name
    Anti-DLL4 APC
  • Delivery Days Customer
    7
  • Application Supplier Note
    Flow cytometry: Recommended dilution: 1-5 microg/ml.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clone ID
    MHD4-46
  • Concentration
    0.1 mg/ml
  • Conjugate
    APC (Allophycocyanin)
  • Isotype
    IgG1
  • Scientific Description
    DLL4 (Delta-like 4) is one of five ligands of Notch receptors. It is up-regulated at sites of physiological and pathological angiogenesis, whereas its expression is low in most adult normal tissues. It is also highly expressed in human clear-cell renal carcinomas, bladder cancers, and breast cancers. Blocking the DLL4-Notch interaction seems to be a promissing therapeutic approach.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DLL4 using anti-DLL4 antibody (A00875-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLL4 antigen affinity purified polyclonal antibody (Catalog # A00875-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLL4 at approximately 80 kDa. The expected band size for DLL4 is at 75 kDa.
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