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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules.

Anti-DNAJA2 Antibody

HPA060538
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetDNAJA2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DNAJA2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10294
  • Target name
    DNAJA2
  • Target description
    DnaJ heat shock protein family (Hsp40) member A2
  • Target synonyms
    cell cycle progression 3 protein; cell cycle progression restoration gene 3 protein; CPR3; DJ3; DJA2; DNAJ; DnaJ (Hsp40) homolog, subfamily A, member 2; dnaJ homolog subfamily A member 2; DNJ3; HIRA interacting protein 4; HIRIP4; PRO3015; RDJ2; renal carcinoma antigen NY-REN-14
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60884
  • Protein Name
    DnaJ homolog subfamily A member 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western blot All lanes: DNAJA2 antibody at 3.79ug/ml Lane 1: A375 whole cell lysate Lane 2: PC-3 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa
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Figure 1. Western blot analysis of DNAJA2 using anti-DNAJA2 antibody (A08918-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse NIH-3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAJA2 antigen affinity purified polyclonal antibody (Catalog # A08918-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNAJA2 at approximately 46KD. The expected band size for DNAJA2 is at 46KD.
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