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Anti-DVL2 Antibody

144-66425
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetDVL2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-DVL2 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID1856
  • Target name
    DVL2
  • Target description
    dishevelled segment polarity protein 2
  • Target synonyms
    segment polarity protein dishevelled homolog DVL-2, dishevelled 2 (homologous to Drosophila dsh), dishevelled, dsh homolog 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14641
  • Protein Name
    Segment polarity protein dishevelled homolog DVL-2
  • Scientific Description
    DVL2 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Dishevelled 2 using anti-Dishevelled 2 antibody (A02404-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dishevelled 2 antigen affinity purified polyclonal antibody (Catalog # A02404-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dishevelled 2 at approximately 79KD. The expected band size for Dishevelled 2 is at 79KD.
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