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Phospho-Dishevelled 2 (Ser143) Antibody (YA2688, Rabbit)

HY-P82943
MedChem Express
TargetDVL2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Phospho-Dishevelled 2 (Ser143) Antibody (YA2688, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID1856
  • Target name
    DVL2
  • Target description
    dishevelled segment polarity protein 2
  • Target synonyms
    dishevelled 2 (homologous to Drosophila dsh); dishevelled, dsh homolog 2; segment polarity protein dishevelled homolog DVL-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Phospho-Dishevelled 2 (Ser143) Antibody (YA2688) is a non-conjugated IgG antibody, targeting Phospho-Dishevelled 2 (Ser143), with a predicted molecular weight of 79 kDa (observed band size: 90-95 kDa). Phospho-Dishevelled 2 (Ser143) Antibody (YA2688) can be used for WB experiment in human, mouse background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Dishevelled 2 using anti-Dishevelled 2 antibody (A02404-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dishevelled 2 antigen affinity purified polyclonal antibody (Catalog # A02404-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dishevelled 2 at approximately 79KD. The expected band size for Dishevelled 2 is at 79KD.
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