Anti-ED-B domain of fibronectin [BC-1]

Absolute Antibody

Anti-ED-B domain of fibronectin [BC-1]

9

This antibody is selective for a cryptic epitope in domain 7 of the B-form splice variant ED-B sequence of fibronectin (EDB-FN). This antibody does not react with plasma fibronectin or thermolysin-digested fibronectin from Wi-38 or Wi-38Val3 cells. Fibron

This antibody is the recombinant humanized version of original anti-fibronectin mouse antibody BC-1. The binding specificity of this antibody for fibronectins was determined using western blotting and radioimmunoassay. This antibody showed strong reaction with WI-38VA13 FN fragments, negative reaction with plasma FN fragments, and a barely detectable reaction with WI-38 FN fragments in a western blot. This antibody was used in the immunohistochemical analysis of the distribution of the ED-B-containing fibronectin isoform (B-FN) in human tissues. The results obtained indicated that fibronectin has a widespread distribution in normal and adult human tissues but the B-FN isoform is restricted only to synovial cells, to some vessels and areas of the interstitium of the ovary, and to the myometrium. Additionally, the B-FN isoform is restricted only to synovial cells, to some vessels and areas of the interstitium of the ovary, and to the myometrium (PMID: 2646306). B-FN isoform is highly expressed during angiogenesis both in neoplastic and in normal tissues and can serve as a marker for formation of new vessels. This antibody can be used for evaluating the level of the angiogenetic process in different neoplasms (PMID: 7525495). This antibody detects human oncofetal fibronectin, which has extremely restricted distribution in normal adult tissues and is highly expressed in fetal and tumor tissues. The biodistribution of 125 Iodine labelled BC-1 was studied in nude mice bearing subcutaneous human tumor implants of U87MG high-grade astrocytoma and SKMel28 melanoma. The results obtained suggested that radiolabeled antibody has tumor targeting potential in vivo and can be a useful marker of neo angiogenesis induced by cancer (PMID: 9406686).

Western Blot, ImmunoHistoChemistry, RadioImmunoAssay

Research Use Only

Monoclonal

BC-1

FN1

fibronectin 1

CIG; cold-insoluble globulin; ED-B; epididymis secretory sperm binding protein; fibronectin; FINC; FN; FNZ; GFND; GFND2; LETS; migration-stimulating factor; MSF; SMDCF

Human

IgG1

Fibronectin

Human

-20°C,2°C to 8°C

12352203