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Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

Anti-EHD1 Antibody

HPA067747
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetEHD1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-EHD1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10938
  • Target name
    EHD1
  • Target description
    EH domain containing 1
  • Target synonyms
    H-PAST, HPAST1, PAST, PAST1, EH domain-containing protein 1, PAST homolog 1, testilin
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Flow Cytometry analysis of U20S cells using anti-EHD1 antibody (A02168-1). Overlay histogram showing U20S cells stained with A02168-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EHD1 Antibody (A02168-1,1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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