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Immunohistochemical staining of human oral mucosa shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human oral mucosa shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human oral mucosa shows strong cytoplasmic positivity in squamous epithelial cells.

Anti-EIF2S2 Antibody

HPA041332
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetEIF2S2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-EIF2S2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8894
  • Target name
    EIF2S2
  • Target description
    eukaryotic translation initiation factor 2 subunit beta
  • Target synonyms
    EIF2, EIF2B, EIF2beta, PPP1R67, eIF-2-beta, eukaryotic translation initiation factor 2 subunit 2, eIF2-beta, eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa, protein phosphatase 1, regulatory subunit 67
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20042
  • Protein Name
    Eukaryotic translation initiation factor 2 subunit 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of EIF2S2 using anti-EIF2S2 antibody (PA2157). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF2S2 antigen affinity purified polyclonal antibody (Catalog # PA2157) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF2S2 at approximately 38 kDa. The expected band size for EIF2S2 is at 38 kDa.
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