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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

Anti-EIF4A2 Antibody

HPA068286
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetEIF4A2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-EIF4A2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1974
  • Target name
    EIF4A2
  • Target description
    eukaryotic translation initiation factor 4A2
  • Target synonyms
    ATP-dependent RNA helicase eIF4A-2; BM-010; DDX2B; EIF4A; eIF-4A-II; eIF4A-II; EIF4F; eukaryotic initiation factor 4A-II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14240
  • Protein Name
    Eukaryotic initiation factor 4A-II
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 2. IHC analysis of eIF4A2 using anti-eIF4A2 antibody (PB9758). eIF4A2 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-eIF4A2 Antibody (PB9758) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with eIF4A2 Polyclonal Antibody, Unconjugated (bs-14549R) at 1:400 overnight at 4°C, DAB staining.
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