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Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with eIF4A2 Polyclonal Antibody, Unconjugated (bs-14549R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with eIF4A2 Polyclonal Antibody, Unconjugated (bs-14549R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with eIF4A2 Polyclonal Antibody, Unconjugated (bs-14549R) at 1:400 overnight at 4°C, DAB staining.

eIF4A2 Polyclonal Antibody

BS-14549R
Bioss Antibodies
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetEIF4A2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    eIF4A2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1974
  • Target name
    EIF4A2
  • Target description
    eukaryotic translation initiation factor 4A2
  • Target synonyms
    ATP-dependent RNA helicase eIF4A-2; BM-010; DDX2B; EIF4A; eIF-4A-II; eIF4A-II; EIF4F; eukaryotic initiation factor 4A-II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14240
  • Protein Name
    Eukaryotic initiation factor 4A-II
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 2. IHC analysis of eIF4A2 using anti-eIF4A2 antibody (PB9758). eIF4A2 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-eIF4A2 Antibody (PB9758) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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