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Western Blot Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, 293 whole cell lysate All lanes: EIF4A2 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, 293 whole cell lysate All lanes: EIF4A2 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, 293 whole cell lysate All lanes: EIF4A2 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa

EIF4A2 Antibody

CSB-PA00384A0RB
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetEIF4A2
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Overview

  • Supplier
    Cusabio
  • Product Name
    EIF4A2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1974
  • Target name
    EIF4A2
  • Target description
    eukaryotic translation initiation factor 4A2
  • Target synonyms
    ATP-dependent RNA helicase eIF4A-2; BM-010; DDX2B; EIF4A; eIF-4A-II; eIF4A-II; EIF4F; eukaryotic initiation factor 4A-II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14240
  • Protein Name
    Eukaryotic initiation factor 4A-II
  • Scientific Description
    Eukaryotic initiation factor 4A plays an important role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins. Two highly homologous forms of functional EIF4A genes, Eif4a1 and Eif4a2, have been isolated in mice; yeast cells also possess 2 EIF4A genes, TIF1 and TIF2. The murine Eif4a and yeast TIF genes appear to belong to a DEAD-box gene family, whose members exhibit extensive amino acid similarity and contain the asp-glu-ala-asp (DEAD) sequence. DEAD-box genes have been identified in species ranging from E-coli to humans. Their function appears to be related to transcriptional/translational regulation (referenced from OMIM).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 2. IHC analysis of eIF4A2 using anti-eIF4A2 antibody (PB9758). eIF4A2 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-eIF4A2 Antibody (PB9758) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with eIF4A2 Polyclonal Antibody, Unconjugated (bs-14549R) at 1:400 overnight at 4°C, DAB staining.
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