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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules.

Anti-ELAVL1 Antibody

HPA046298
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetELAVL1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ELAVL1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1994
  • Target name
    ELAVL1
  • Target description
    ELAV like RNA binding protein 1
  • Target synonyms
    ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R); ELAV1; ELAV-like protein 1; embryonic lethal, abnormal vision, drosophila, homolog-like 1; Hu antigen R; Hua; hu-antigen R; HUR; MelG
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15717
  • Protein Name
    ELAV-like protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ELAVL1 using anti-ELAVL1 antibody (M00736). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat spleen tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELAVL1 antigen affinity purified monoclonal antibody (Catalog # M00736) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ELAVL1 at approximately 36 kDa. The expected band size for ELAVL1 is at 36 kDa.
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