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Anti-EphA3 [KB004]

Ab02488-1.1
Absolute Antibody
ApplicationsFunctional Assay
Product group Antibodies
TargetEPHA3
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-EphA3 [KB004]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody has been shown to have a wide therapeutic potential. Human version of this antibody was shown to be safe for treatment of patients with refractory hematologic malignancies (Swords et al, 2016; pmid:27736729). Further, it has been shown that this antibody was cytotoxic to LK63 cell lines (Charmsaz et al, 2017; pmid:27922598). Besides this, the antibody was also shown to reduce multiple myeloma angiogenesis (Caivano et al, 2017; pmid:28415715). Fianlly, this antibody has been observed to reduice lung fibrosis is mice (WO2018053032).
  • Applications
    Functional Assay
  • Applications Supplier
    therapeutic; functional assay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    KB004
  • Gene ID2042
  • Target name
    EPHA3
  • Target description
    EPH receptor A3
  • Target synonyms
    EK4; EPH-like kinase 4; eph-like tyrosine kinase 1; ephrin type-A receptor 3; ETK; ETK1; HEK; HEK4; human embryo kinase 1; testicular tissue protein Li 64; TYRO4; TYRO4 protein tyrosine kinase; tyrosine-protein kinase receptor ETK1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP29320
  • Protein Name
    Ephrin type-A receptor 3
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This antibody was created by humanizing the IIIA4 antibody. The IIIA4 antibody was raised by immunizing mice with LK63 cells.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Eph Receptor A3/EPHA3 using anti-Eph Receptor A3/EPHA3 antibody (A02872-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eph Receptor A3/EPHA3 antigen affinity purified polyclonal antibody (Catalog # A02872-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eph Receptor A3/EPHA3 at approximately 135 kDa. The expected band size for Eph Receptor A3/EPHA3 is at 110 kDa.
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Lane 1: H9C2 cell lysates probed with Eph receptor A3 Polyclonal Antibody, Unconjugated (bs-7032R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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