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Western Blot analysis of JK cells using EphA3 Polyclonal Antibody
Western Blot analysis of JK cells using EphA3 Polyclonal Antibody
Western Blot analysis of JK cells using EphA3 Polyclonal Antibody

EPHA3 Antibody

CSB-PA008057
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetEPHA3
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Overview

  • Supplier
    Cusabio
  • Product Name
    EPHA3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2042
  • Target name
    EPHA3
  • Target description
    EPH receptor A3
  • Target synonyms
    EK4; EPH-like kinase 4; eph-like tyrosine kinase 1; ephrin type-A receptor 3; ETK; ETK1; HEK; HEK4; human embryo kinase 1; testicular tissue protein Li 64; TYRO4; TYRO4 protein tyrosine kinase; tyrosine-protein kinase receptor ETK1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29320
  • Protein Name
    Ephrin type-A receptor 3
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Eph Receptor A3/EPHA3 using anti-Eph Receptor A3/EPHA3 antibody (A02872-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eph Receptor A3/EPHA3 antigen affinity purified polyclonal antibody (Catalog # A02872-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eph Receptor A3/EPHA3 at approximately 135 kDa. The expected band size for Eph Receptor A3/EPHA3 is at 110 kDa.
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Lane 1: H9C2 cell lysates probed with Eph receptor A3 Polyclonal Antibody, Unconjugated (bs-7032R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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