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Immunohistochemical staining of human skin shows distinct cytoplasmic positivity in keratinocytes.
Immunohistochemical staining of human skin shows distinct cytoplasmic positivity in keratinocytes.
Immunohistochemical staining of human skin shows distinct cytoplasmic positivity in keratinocytes.

Anti-EPHB3 Antibody

HPA007698
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetEPHB3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-EPHB3
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2049
  • Target name
    EPHB3
  • Target description
    EPH receptor B3
  • Target synonyms
    EK2, ETK2, HEK2, TYRO6, ephrin type-B receptor 3, EPH-like tyrosine kinase 2, embryonic kinase 2, human embryo kinase 2, tyrosine-protein kinase TYRO6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP54753
  • Protein Name
    Ephrin type-B receptor 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of EPHB3 using anti-EPHB3 antibody (A04659). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse stomach tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPHB3 antigen affinity purified polyclonal antibody (Catalog # A04659) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPHB3 at approximately 110 kDa. The expected band size for EPHB3 is at 110-130 kDa.
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