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anti-EPO Receptor antibody

ARG55576
Arigo Biolaboratories
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
TargetEPOR
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Overview

  • Supplier
    Arigo Biolaboratories
  • Product Name
    anti-EPO Receptor antibody
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2057
  • Target name
    EPOR
  • Target description
    erythropoietin receptor
  • Target synonyms
    EPO-R, erythropoietin receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EPO Receptor using anti-EPO Receptor antibody (A00427-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPO Receptor antigen affinity purified polyclonal antibody (Catalog # A00427-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPO Receptor at approximately 55KD. The expected band size for EPO Receptor is at 55KD.
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