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Immunohistochemical staining of human salivary gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human salivary gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human salivary gland shows strong cytoplasmic positivity in glandular cells.

Anti-EPRS Antibody

HPA026490
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetEPRS1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-EPRS
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2058
  • Target name
    EPRS1
  • Target description
    glutamyl-prolyl-tRNA synthetase 1
  • Target synonyms
    EARS, EPRS, GLUPRORS, HLD15, PARS, PIG32, QARS, QPRS, bifunctional glutamate/proline--tRNA ligase, bifunctional aminoacyl-tRNA synthetase, cell proliferation-inducing gene 32 protein, glutamate tRNA ligase, glutamatyl-prolyl-tRNA synthetase, glutaminyl-tRNA synthetase, proliferation-inducing gene 32 protein, proliferation-inducing protein 32, proline-tRNA ligase, prolyl-tRNA synthetase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07814
  • Protein Name
    Bifunctional glutamate/proline--tRNA ligase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of EPRS1/PARS using anti-EPRS1/PARS antibody (A02967-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hek293 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat pancreas tissue lysates, Lane 5: mouse pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPRS1/PARS antigen affinity purified polyclonal antibody (Catalog # A02967-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPRS1/PARS at approximately 170-180KD. The expected band size for EPRS1/PARS is at 171KD.
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