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Immunohistochemical staining of human kidney shows moderate nuclear positivity in cells in tubules and cells in glomeruli.
Immunohistochemical staining of human kidney shows moderate nuclear positivity in cells in tubules and cells in glomeruli.
Immunohistochemical staining of human kidney shows moderate nuclear positivity in cells in tubules and cells in glomeruli.

Anti-ERG Antibody

HPA046598
Atlas Antibodies
ApplicationsImmunoHistoChemistry, Chip-exoSeq
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetERG
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ERG
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry, Chip-exoSeq
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2078
  • Target name
    ERG
  • Target description
    ETS transcription factor ERG
  • Target synonyms
    ERG, ETS transcription factor; erg-3; erythroblast transformation-specific transcription factor ERG variant 10; ets-related; FUS/ERG fusion protein; p55; TMPRSS2/ERG fusion; transcriptional regulator ERG; transcriptional regulator ERG (transforming protein ERG); v-ets avian erythroblastosis virus E26 oncogene homolog; v-ets avian erythroblastosis virus E26 oncogene related; v-ets erythroblastosis virus E26 oncogene homolog; v-ets erythroblastosis virus E26 oncogene like
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11308
  • Protein Name
    Transcriptional regulator ERG
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ERG using anti-ERG antibody (A00793-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Colo320 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERG antigen affinity purified polyclonal antibody (Catalog # A00793-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERG at approximately 55 kDa. The expected band size for ERG is at 55 kDa.
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