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Immunoprecipitating ERG in Rat heart tissue Lane 1: Rabbit control IgG (1microg) instead of CSB-PA007781HA01HU in Rat heart tissue. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA007781HA01HU (8microg) + Rat heart tissue (500microg) Lane 3: Rat heart tissue (10microg)
Immunoprecipitating ERG in Rat heart tissue Lane 1: Rabbit control IgG (1microg) instead of CSB-PA007781HA01HU in Rat heart tissue. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA007781HA01HU (8microg) + Rat heart tissue (500microg) Lane 3: Rat heart tissue (10microg)
Immunoprecipitating ERG in Rat heart tissue Lane 1: Rabbit control IgG (1microg) instead of CSB-PA007781HA01HU in Rat heart tissue. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA007781HA01HU (8microg) + Rat heart tissue (500microg) Lane 3: Rat heart tissue (10microg)

ERG Antibody

CSB-PA007781HA01HU
Cusabio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetERG
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Overview

  • Supplier
    Cusabio
  • Product Name
    ERG Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2078
  • Target name
    ERG
  • Target description
    ETS transcription factor ERG
  • Target synonyms
    LMPHM14, erg-3, p55, transcriptional regulator ERG, ERG, ETS transcription factor, FUS/ERG fusion protein, TMPRSS2/ERG fusion, ets-related, transcriptional regulator ERG (transforming protein ERG), v-ets avian erythroblastosis virus E26 oncogene homolog, v-ets avian erythroblastosis virus E26 oncogene related, v-ets erythroblastosis virus E26 oncogene homolog, v-ets erythroblastosis virus E26 oncogene like
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11308
  • Protein Name
    Transcriptional regulator ERG
  • Scientific Description
    Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ERG using anti-ERG antibody (A00793-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Colo320 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERG antigen affinity purified polyclonal antibody (Catalog # A00793-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERG at approximately 55 kDa. The expected band size for ERG is at 55 kDa.
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