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Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Anti-ERN2 Antibody

HPA076875
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetERN2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ERN2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10595
  • Target name
    ERN2
  • Target description
    endoplasmic reticulum to nucleus signaling 2
  • Target synonyms
    IRE1-BETA, IRE1b, IRE2p, hIRE2p, serine/threonine-protein kinase/endoribonuclease IRE2, ER to nucleus signalling 2, IRE1 beta, IRE1, S. cerevisiae, homolog of, endoplasmic reticulum to nucleus signalling 2, inositol-requiring 1 beta, inositol-requiring protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ76MJ5
  • Protein Name
    Serine/threonine-protein kinase/endoribonuclease IRE2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of ERN2 using anti-ERN2 antibody (A05659-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HCCT tissue lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERN2 antigen affinity purified polyclonal antibody (Catalog # A05659-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERN2 at approximately 102 kDa. The expected band size for ERN2 is at 102 kDa.
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