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ICC/IF analysis of A549 cells using GTX87426 IRE1 beta antibody. The picture on the right is blocked with the synthesized peptide.
ICC/IF analysis of A549 cells using GTX87426 IRE1 beta antibody. The picture on the right is blocked with the synthesized peptide.
ICC/IF analysis of A549 cells using GTX87426 IRE1 beta antibody. The picture on the right is blocked with the synthesized peptide.

IRE1 beta antibody

GTX87426
GeneTex
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetERN2
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Overview

  • Supplier
    GeneTex
  • Product Name
    IRE1 beta antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    ICC/IF: 1:100~1:500. IHC-P: 1:50~1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10595
  • Target name
    ERN2
  • Target description
    endoplasmic reticulum to nucleus signaling 2
  • Target synonyms
    IRE1-BETA, IRE1b, IRE2p, hIRE2p, serine/threonine-protein kinase/endoribonuclease IRE2, ER to nucleus signalling 2, IRE1 beta, IRE1, S. cerevisiae, homolog of, endoplasmic reticulum to nucleus signalling 2, inositol-requiring 1 beta, inositol-requiring protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ76MJ5
  • Protein Name
    Serine/threonine-protein kinase/endoribonuclease IRE2
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Dai F, Dong S, Rong Z, et al. Expression of inositol-requiring enzyme 1β is downregulated in azoxymethane/dextran sulfate sodium-induced mouse colonic tumors. Exp Ther Med. 2019,17(4):3181-3188. doi: 10.3892/etm.2019.7317
    Read this paper

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Figure 1. Western blot analysis of ERN2 using anti-ERN2 antibody (A05659-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HCCT tissue lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERN2 antigen affinity purified polyclonal antibody (Catalog # A05659-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERN2 at approximately 102 kDa. The expected band size for ERN2 is at 102 kDa.
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