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ProductsBack/Goat anti-ERN2 / IRE1b

Goat anti-ERN2 / IRE1b

EB08560
Everest Biotech
ApplicationsELISA
Product group Antibodies
TargetERN2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ERN2 / IRE1b Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA
  • Applications Supplier
    Pep-ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID10595
  • Target name
    ERN2
  • Target description
    endoplasmic reticulum to nucleus signaling 2
  • Target synonyms
    IRE1-BETA, IRE1b, IRE2p, hIRE2p, serine/threonine-protein kinase/endoribonuclease IRE2, ER to nucleus signalling 2, IRE1 beta, IRE1, S. cerevisiae, homolog of, endoplasmic reticulum to nucleus signalling 2, inositol-requiring 1 beta, inositol-requiring protein 2
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_150296.3. Purification: Antigen affinity purified. Names and symbols: ERN2; endoplasmic reticulum to nucleus signaling 2 ; IRE1b; ER to nucleus signalling 2; IRE1 beta; IRE1, S. cerevisiae, homolog of; endoplasmic reticulum to nucleus signalling 2; inositol-requiring 1 beta
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ERN2 using anti-ERN2 antibody (A05659-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HCCT tissue lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERN2 antigen affinity purified polyclonal antibody (Catalog # A05659-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERN2 at approximately 102 kDa. The expected band size for ERN2 is at 102 kDa.
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