Anti-estrogen receptor alpha [D547]

Absolute Antibody

Anti-estrogen receptor alpha [D547]

9

This antibody is specific for human estrogen receptor. The estrogen receptor is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differe

crude nuclear E*R of calf uterus (0.1 pmol), normal rat serum (10 Ml), and hybridoma medium were used in a double antibody immunoprecipitation with this antibody (Greene et al, 1980; pmid:6928610). ELISA was preformed with this antibody on human MCF-7 cell cytosol (Greene et al, 1982; pmid:6979662). Fresh breast cancer tumor was immunostained using this antibody (Miller et al, 1993; pmid:8490902). An EIA was preformed on cytosol from breast cancer tumors using this antibody (Cren et al, 1991; pmid:1911441). COS-7 cells cotransfected with expression plasmids encoding ERa and HA-tagged ERb were immunoprecipitated with this antibody (Ogawa et al, 1998; pmid:9473491). Sucrose Density Gradient Analysis was preformed on GH3 cells (Tate et al, 1984; pmid:6708522). Immunocytochemistry was preformed on the reproductive tract of Female cynomolgus macaques using this antibody (McClellan et al, 1984; pmid:6373239). [3H]E2-labeled macaque E2 receptors were examined on sucrose density gradients using this antibody (McClellan et al, 1984; pmid:6373239).

Functional Assay, ImmunoFluorescence, ImmunoPrecipitation, ELISA, ImmunoHistoChemistry

Research Use Only

Monoclonal

D547

ESR1

estrogen receptor 1

E2 receptor alpha; ER; Era; ER-alpha; ESR; ESRA; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; ESTRR; NR3A1; nuclear receptor subfamily 3 group A member 1; oestrogen receptor alpha

Human

IgG1

Estrogen receptor

This chimeric human antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203