Anti-estrogen receptor alpha [D75]

Absolute Antibody

Anti-estrogen receptor alpha [D75]

9

This antibody is specific for human estrogen receptor. The estrogen receptor is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differe

Crude nuclear E*R of calf uterus (0.1 pmol), normal rat serum (10 Ml), and hybridoma medium were used in a double antibody immunoprecipitation with this antibody (Greene et al, 1980; pmid:6928610). ELISA was preformed with this antibody on human MCF-7 cell cytosol (Greene et al, 1982; pmid:6979662). Fresh breast cancer tumor was immunostained using this antibody (Miller et al, 1993; pmid:8490902). Sucrose Density Gradient Analysis was preformed on an extract made from MCF-7 human breast cancer cell line using this antibody (Wong et al, 1991; pmid:1705046). This antibody was used for immunohistochemistry on human breeast cancer (Wong et al, 1991; pmid:1705046). ELISA was preformed on human tumor whole-cell extract using this antibody (Scott et al, 1991; pmid:1864980). An immunohistochemistry was preformed on human breast cancer tissue using this antibody (Miller et al, 1993; pmid:8490902). Immunohistochemistry was preformed on tumor cells from humans with recurrent breast cancer. For this experiment this antibody was used (Rasmussen and Kamby, 1989; pmid:2616369). Western blot was preformed on SV40-transformed HBEC, and MCF-7, T47D, MDA-MB-231 cells using this antibody (Kang et al, 1997; pmid:9054615). Extracts from infected Sf9 cells were analyzed by Western blotting using this antibody (Beekman et al, 1993; pmid:8264659). Gel shift was preformed on human breast tumor sample and control MCF-7 extracts using this antibody (Montgomery et al, 1993; pmid:8219255).

Functional Assay, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, Other Application

Research Use Only

Monoclonal

D75

ESR1

estrogen receptor 1

E2 receptor alpha; ER; Era; ER-alpha; ESR; ESRA; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; ESTRR; NR3A1; nuclear receptor subfamily 3 group A member 1; oestrogen receptor alpha

Human

IgG1

Estrogen receptor

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203