Anti-estrogen receptor alpha [H222.2]

Absolute Antibody

Anti-estrogen receptor alpha [H222.2]

9

This antibody is specific for human estrogen receptor. The estrogen receptor is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differe

ELISA was preformed on human breast cancer tissue, human,monkey and calf uterus tissue and hen oviduct tissue. For this ELISA this antibody was used (Greene et al, 1984; pmid:6200700). Semi-confluent HeLa cells (3-5 x 106 cells/9 cm Petri dish) transfected with 10 ug of recombinant DNA (HEO-HE14) were immunoprecipitated with this antibody (Kumar et al, 1986; pmid:3780678). Immunohistochemistry was preformed, to compare them to their own antibodies, on human breast cancers using this antibody (Saati et al , 1993; pmid:8406995). Western blot was preformed on protein extracts from (i) transformed E. coli; (ii) COS cells transfected with pSG5-HGO or pSG5-HG19 (containing a deletion mutant of the ER cDNA lacking the sequence coding for the 178 N terminal amino-acids) the human breast-cancer cell line MCF-7 (ATCC HTB 22, Rockville, MD) and (iv) endometrial tissue. In this western blotting this antibody was used (Saati et al , 1993; pmid:8406995). This antibody was used for immunohistochemistry on brain tissue of female rats (Henry Jr et al, 1991; pmid:1917572). Immunohistochemistry was preformed on tissue samples from surgically removed human uteri using this antibody (Charpin et al, 1986; pmid:3522449). Sucrose density gradients were preformed on anterior oviduct or liver tissue from a red-sided garter snake using this antibody (Whittier et al, 1991; pmid:2026308). A steroid specificty trail was preformed on anterior oviduct or liver tissue from a red-sided garter snake using this antibody (Whittier et al, 1991; pmid:2026308). to characterize the putative membrane ER on human spermatozoa, western blot was preformed on human sperm lysates (Luconi et al, 2004; pmid:15288769).

Functional Assay, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry

Research Use Only

Monoclonal

H222.2

ESR1

estrogen receptor 1

E2 receptor alpha; ER; Era; ER-alpha; ESR; ESRA; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; ESTRR; NR3A1; nuclear receptor subfamily 3 group A member 1; oestrogen receptor alpha

Rat

IgG1

Estrogen receptor

Bovine, Chicken, Human, Monkey, Reptile, Rat

-20°C,2°C to 8°C

12352203