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Anti-ETF1 Antibody

144-05920
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetETF1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-ETF1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2107
  • Target name
    ETF1
  • Target description
    eukaryotic translation termination factor 1
  • Target synonyms
    D5S1995, ERF, ERF1, RF1, SUP45L1, TB3-1, eukaryotic peptide chain release factor subunit 1, polypeptide chain release factor 1, protein Cl1, sup45 (yeast omnipotent suppressor 45) homolog-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62495
  • Protein Name
    Eukaryotic peptide chain release factor subunit 1
  • Scientific Description
    ETF1 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ETF1 using anti-ETF1 antibody (A04157-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse NIH3T3 whole cell lysates, Lane 4: mouse RAW246.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ETF1 antigen affinity purified polyclonal antibody (Catalog # A04157-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ETF1 at approximately 49KD. The expected band size for ETF1 is at 49KD.
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